Improved Bioassay of Xylella fastidiosa Using Nicotiana tabacum Cultivar SR1
نویسندگان
چکیده
منابع مشابه
Mapping analysis of the Xylella fastidiosa genome.
A cosmid library was made of the 2.7 Mb genome of the Gram-negative plant pathogenic bacterium Xylella fastidiosa and analysed by hybridisation mapping. Clones taken from the library as well as genomic restriction fragments of rarely cutting enzymes were used as probes. The latter served as a backbone for ordering the initial map contigs and thus facilitated gap closure. Also, the co-linearity ...
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The use of tobacco dates back to the ancient civilizations of the Americas, where it played a central role in religious occasions. The peoples smoked tobacco in cigars and pipes and chewed it with lime, for its pleasurable euphoriant effects. In the 16 th century, Europeans spread the use of tobacco in North America, while the Spanish bring it into Europe. In the 1559, Jean Nicot, the French am...
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Xylella fastidiosa (Xf) is a fastidious gram-negative, xylem-limited bacterium that causes diseases in many crops of economic importance, such as grape (Pierce’s disease), almond, peach, coffee, plum and citrus. Xf can infect all known Citrus sinensis cultivars and causes Citrus variegated chlorosis (CVC). One major endophyte isolated from CVC-asymptomatic plants is the bacterium Curtobacterium...
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We have developed an autonomously replicating Xylella fastidiosa (Xf)/E. coli plasmid that efficiently transforms Xf; unfortunately this plasmid was not stably maintained in Xf cells en planta or without antibiotic selection in vitro. Another plasmid, containing an Xf native plasmid, a Kan cassette and cloned in pUC18 was also constructed and shown to be unstable without antibiotic selection. 1...
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The present study investigates antibacterial activity of tobacco extracts from Nicotiana tabacum at different concentrations in different polar solvents. Six different extracts were prepared, using 5 different polar solvents viz., Ethanol, Ethyl acetate, n-Hexane, Acetone, Butanol and water. Four different concentrations (6, 12, 18 and 24 mg of sample disc) of each extract were subjected for pr...
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ژورنال
عنوان ژورنال: Plant Disease
سال: 2008
ISSN: 0191-2917,1943-7692
DOI: 10.1094/pdis-92-1-0014